The Multiple Homogenizer (originally designed for insect samples)
allows simultaneous preparation of 96 tissue samples, reducing
homogenization time by a factor of 10-fold. Up to 3000 samples
for immuno-assay analysis may be prepared by one person daily.
Earlier labour-intensive and time-consuming methods have employed
the use of standard micro-centrifuge tubes with close fitting
glass rods. These methods proved to be the limitation in sample
preparation when quantifying the activity of the enzyme responsible
for the resistance to insecticides in aphids.
The illustration shows the precision component parts and the
method of assembly. When buffer and samples have been loaded
into the wells of the microplate the multi-pestle is inserted
and rotated manually in an orbital motion.
The volume lost after this process regardless of homogenization
volume was only 5µl when the pestles were extracted carefully.
Thus 50% and 90% of sample was recovered reliably when homogenized
in 10µl and 50µl respectively.
Other items of equipment for the analytical process include
a special electrophoresis gel comb (corresponding to the pitches
of the microplate), an 8-position multi-pipette, electrophoresis
cabinet and power supply.
Reference: R. H. ffrench-Constant/A. L. Devonshire Biochemical
Genetics Vol. 25 Nos. 7/8 1987.
Article: A Multiple
Homogenizer for the rapid preparation of samples for immunoassays